Copper-tin nanocomposites-loaded exosomes persuaded skin carcinoma cell apoptosis and arrested cell cycle

Author:

Abd-Rabou Ahmed A.1,Kishta Mohamed S.1,Yakout Saad M.2,Youssef Ahmed M.2,Abdallah Ahmed N.1,Ahmed Hanaa H.1

Affiliation:

1. 1Hormones Department, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Cairo, Egypt

2. Inorganic Chemistry Department, National Research Centre, Dokki, Cairo, Egypt

Abstract

Abstract Skin cancer is one of the most prevalent ailments in men that may be provoked by sun exposure. The chemotherapeutic potential of copper (Cu)- and tin (Sn)-based substances against a range of cancers has been studied. Based on the differences in how normal and tumour cells responded, they were utilized as targeted anticancer drugs. In the current study, nanotechnology has revolutionized cancer therapy by introducing Cu and Sn nanocomposites to overcome the drawbacks of the conventional chemotherapy and radiation therapy in terms of effectiveness and adverse effects due to uneven distribution and cytotoxicity. CuS/SnS nanocomposites (S1, S2, S3) were synthesized and characterized, then encapsulated into exosomes (Exo) isolated from bone marrow-derived mesenchymal stem cells (BM-MSCs) and characterized once again. S1-Exo, S2-Exo, and S3-Exo were investigated biologically using cytotoxicity, apoptosis, and cell cycle assays. The The X-ray diffractions analysis of CuS/SnS nanonanocomposites were indexed to hexagonal CuS structure and orthorhombic α-SnS phase. The TEM images of S1 and S3 nanocomposites showed the presence of nanorods particles with average length of 80 nm and diameter of 16 nm. S1-Exo showed superior cytotoxic effect against A431 skin cancerous cells than the free nano-form S1. It was intriguingly that S1-Exo recorded 1.109 times more than DOX in its anticancer activity. Flow cytometry showed that S1-Exo recorded 40.2% early apoptosis and 22.1% late apoptosis. In conclusion, the CuS/SnS nanocomposites loaded into exosomes could be of great potential as anti-skin cancer candidate through induction of cell death-mediated apoptosis

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3