Molecular identification of yeasts in oral cavity in immunocompetent teenagers from the North of Mexico

Author:

Rojas Olga C.1,Amaral-Montesino Cintia2,Mendoza-Olazaran Soraya2,González-Álvarez Rafael3,Montoya Alexandra M.1

Affiliation:

1. Universidad Autónoma de Nuevo León

2. Universidad de Monterrey

3. Instituto Tecnológico de los Altos de Jalisco

Abstract

Abstract Human oral cavity is normally colonized for microorganisms including bacteria, fungi, archaea, viruses and protozoa. The aim of this study was to determine the frequency of Candida albicans in de oral cavity in a group of medicine students from the north of Mexico. 240 oral samples from healthy students were analyzed by traditional microbiology cultures and DNA sequencing. 57 samples grew in media cultures. Sequence analysis identified the yeast as follow: 28 Clavispora lusitaniae, 20 Candida albicans, 5 Pichia kudriavzevii and 4 Candida parapsilosis. Our finding revealed that 23.75% of healthy population has a potential pathogen in its mouth. Surprisingly Candida albicans is not the predominant yeast, instead of others non-Candida species are the colonizers of the oral cavity in immunocompetent individuals. Oral cavity is colonized by potential pathogen yeast being Clavispora lusitaniae, Candida albicans, Pichia kudriavzevii and Candida parapsilosis.

Publisher

Research Square Platform LLC

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4. Ghannoum, Mahmoud A., Jurevic, Richard J., Mukherjee, Pranab K., Cui, Fan, Sikaroodi, Masoumeh, Naqvi, Ammar, & Gillevet, Patrick M. (2010). Characterization of the Oral Fungal Microbiome (Mycobiome) in Healthy Individuals. PLOS Pathogens, 6(1), e1000713. doi:10.1371/journal.ppat.1000713

5. Wu, Jinyan, Guo, Hong, Yi, Guohui, Zhou, Limin, He, Xiaowen, Huang, Xianxi,.. . Xu, Jianping. (2014). Prevalent drug resistance among oral yeasts from asymptomatic patients in Hainan, China. Mycopathologia, 177(5), 299–307.

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