Isolation and Identification of Cadmium-Reducing Bacteria from Contaminated Coastal Sediment in The Northern Coast of Indramayu, Indonesia

Abstract

Abstract Cadmium (Cd) is one of the heavy metals pollutants and its accumulation impacted the sustainability of marine organisms. The aim of current research was to isolate and identify the cadmium-reducing bacteria from contaminated coastal sediment in Karangsong port, Indramayu, Indonesia. The isolates were investigated for its potential to reduce cadmium showed the cadmium reduction drastically up to 50% at 6 hours treated under different cadmium concentration of 0,5, 1, and 1,5 ppm, respectively. Morphological characteristics were observed in most of isolates. Out of 8 isolates, two selected strains such as Karangsong Cd3 and Cd7 were identified by 16S rRNA sequencing as Pseudoalteromonas issachenkonii strain KMM 3549 (Acc. No. NR 025139.1) and Pseudoalteromonas tetraodonis GFC strain IAM 14160 (Acc. No. NR 041787.1), respectively. The cadmium resistance profile showed that the selected isolates were resistant to various concentration of cadmium (Cd). These results suggest that the Karangsong Cd 3 and Cd 7 could facilitate the new references of future microbial application for bioremediations efforts.