Over-expression of long non-coding RNA LINC00342 as diagnostic biomarker for Kidney Renal Clear Cell Carcinoma

Abstract

Abstract Background: Kidney renal clear cell carcinoma (KIRC), being the most common type of renal cancer, exhibits a high mortality and recurrence rate primarily because a significant number of patients are already in advanced stages at the time of diagnosis. Identifying a biological marker for early-stage KIRC has become a top priority. Recently, some studies have shown that long non-coding RNA LINC00342 promotes the proliferation, invasion, and migration in gastric cancer, colorectal cancer and non-small cell lung cancer through a variety of ways. However, the involvement and mechanism of linc00342 in KIRC is still unclear. The aim of this study was to examine the diagnostic and prognostic value of linc00342 in KIRC, to investigate the effects of linc00342 on the biological functions of KIRC cells, and to explore potential mechanisms of linc00342 in KIRC. Methods: We downloaded the linc00342 expression data and clinical information of KIRC from the TCGA database and constructed a prognostic prediction model. In vitro, the effect of silencing linc00342 on KIRC cell proliferation, apoptosis, metastasis, and invasion was measured by colony-formation assay, flow cytometric analysis, wound-healing assay and Transwell assay, respectively. Results: Firstly, our predictive model was established by using TCGA database. Secondly, Linc00342 was involved in various biological functions of KIRC by using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Thirdly, In vitro, linc00342 was overexpressed in KIRC by RT-qPCR in tissue and cell models. Moreover, we found that linc00342 can inhibit cell apoptosis and promote cell proliferation, invasion, and migration. Conclusion: To our knowledge, our study is the first to construct a nomogram prediction model by combining the gene expression of linc00342 with clinical data, and confirmed that linc00342 can be an independent prognostic factor of KIRC through in vitro validation.