Abstract
Abstract
Herein, we investigated whether L-ascorbic acid and α-tocopherol supplementation has potential to alleviate arsenic induced immunotoxicities in thymus, spleen and circulating leukocytes. Forty-eight adult male Wistar rats were randomly divided into four groups before the treatments. Group-I (control); Group-II (sodium arsenite, 3mg/Kg/day/rat); Group-III [sodium arsenite + L-Ascorbic acid(L-AA) (200mg/Kg/day/rat) and α-tocopherol (α-T) (400mg/Kg/day/rat)]; Group-IV (L-AA and α-T). The result showed that sodium arsenite exposure (consecutive 30 days) caused weight reduction, structural alterations of thymus and spleen, accompanied by decrease in thymocyte and splenocyte counts. Decreased superoxide dismutase and catalase activities, increased malondialdehyde and protein-carbonyl contents, reduced Nrf2 and Bcl2 expression and increased p-ERK, NF-β, Bax, and cleaved-caspase-3 expression were also observed in thymus and spleen of arsenic exposed rat. Enhanced plasma ACTH and corticosterone, ROS induced apoptosis of lymphocytes were also observed. L-AA and α-T supplement has the potential to abrogate the deleterious impact of arsenic on thymus, spleen and circulating lymphocytes. Whole transcriptome analysis of leukocytes revealed that arsenic treatment augmented the expression of Itga4, Itgam, and MMP9 genes, which might help in transient migration of leukocytes through the endothelial cell layer. Supplementation with L-AA and α-T maintained Itga4, Itgam, and MMP9 gene expression within leukocytes at lower level.
Publisher
Research Square Platform LLC
Reference109 articles.
1. Acute arsenic exposure induces inflammatory responses and CD4 + T cell subpopulations differentiation in spleen and thymus with the involvement of MAPK, NF-kB, and Nrf2;Duan Xiaoxu;Molecular Immunology,2017
2. Technologies for Arsenic Removal from Water: Current Status and Future Perspectives;Nicomel Nina;International Journal of Environmental Research and Public Health,2015
3. Islam, Khairul, Qian Qian Wang, and Hua Naranmandura. 2015. Molecular Mechanisms of Arsenic Toxicity. In, 77–107. https://doi.org/10.1016/B978-0-12-802229-0.00002-5.
4. Comparison of the urinary excretion of arsenic metabolites after a single oral dose of sodium arsenite, monomethylarsonate, or dimethylarsinate in man;Buchet JP;International archives of occupational and environmental health,1981
5. The arsenic contamination of rice in Guangdong Province, the most economically dynamic provinces of China: arsenic speciation and its potential health risk;Lin Kai;Environmental Geochemistry and Health,2015