First Systematic Analysis for Chemical Composition by HPLC-ESI-MSn and Antioxidant Activity Comparison of Dendrobium huoshanense and Dendrobium officinale

Abstract

Abstract Background: Dendrobium huoshanense C. Z. Tang et S. J. Cheng (D. huoshanense) is a precious Dao-di herb with high quality, excellent efficacy and edible value of China, Dendrobium officinale Kimura et Migo (D. officinale) is widely utilized and commonly used as a substitute for D. huoshanense. But few studies have been conducted to systematically analyze chemical composition and bioactivities of the two.Methods: HPLC-ESI-MSn was used to identify the compounds in D. huoshanense and D. officinale grown in Huoshan from methanol extracts and different polar fractions of water extracts, including petroleum ether, ethyl acetate and n-butanol; The in vitro antioxidant activity were evaluated by the scavenging effects on DPPH and ·OH radicals, total reduction ability and total antioxidant ability.Results: 61 compounds were identified in D. huoshanense and 49 compounds were identified in D. officinale and with 43 shared compounds; Among them, 44 and 7 compounds were respectively identified from the two for the first time. Oxyresveratrol, quercetin-3-O-glucoside, quercetin-3-O-arabinoside, naringenin-7-O-glucoside, baicalin, hesperetin, vitexin and apigenin-6-C-pentosyl-8-C-(2’’-O-quinoyl)-glucoside were first identified in Dendrobium. In addition, 5 compounds were unique in D. huoshanense compared with other species of Dendrobium . The results of in vitro antioxidant activity showed that n-BuOH-soluble fraction of D. huoshanense and D. officinale both revealed the strongest antioxidant activities compared with other fractions, furthermore, the antioxidant activities of different fractions of D. huoshanense were separately superior to that of D. officinale.Conclusion: These results may provide a reference for the substances study about D. huoshanense's high-quality efficacy, and also provide a scientific basis for its development and utilization.