Identification of key genes associated with alcohol addiction and DNA replication aberrant in Ovarian serous cystadenocarcinoma using an integrated bioinformatics analysis

Abstract

AbstractBackground Multiple evidence indicates a significant correlation between alcohol and DNA replication aberrant in cancer, but the role of this correlation in Ovarian serous cystadenocarcinoma (OSC) remains insufficient. This research evaluated correlation between DNA replication related genes (DRRGs) and alcohol addiction related genes (AARGs) in OSC via bioinformatics Methods Multiple bioinformatics approaches were used to confirm the diagnosis, prognosis, and treatment significance of DRRGs in OSC. The effect of MCM3 on OSC proliferation and DNA replication were confirmed by MTT and EdU analysis. Results the level of ORC2/4, LIG1, RNASEH2B/C, RFC1, POLE4 and POLD4 was significantly decreased in OSC, but other DRRGs was obviously increased in OSC samples compared to normal samples. PCA analysis indicated that these DRRGs could be biomarkers for early diagnosis in OSC. PRIM2, ORC3, POLD1, POLD2, MCM3, RPA2, GMNN and RAD52 were identified as prognostic signatures. High-risk group has a poor prognosis. MCM3 was a key gene in the DRRG and AARGs in the development of OSC, which was enhanced in OSC patients EVs, and promoted the DNA replication and proliferation. Conclusion The hub gene MCM3 represent a significant gene involved in alcohol addiction and DNA replication aberrant for OSC progression.