Single Nucleotide Polymorphisms of NLRP12 Gene and Association with Psoriasis

Abstract

Abstract Background NLRP12 has been authenticated as an important negative regulator in multiple metabolic and inflammatory diseases. Psoriasis is an important inflammatory disease and often suffer from comorbidities such as inflammatory bowel disease, obese, etc. However, the role of NLRP12 in psoriasis remains unexplored. Methods SNP mutation sites of NLRP12 gene were screened in psoriasis and control groups, followed by genotyping and correlation analysis. The expression of NLRP12 gene and protein in blood and tissue samples of psoriasis patients were determined by Quantitative Real-time polymerase chain reaction (qPCR), immunohistochemistry and Western Blot. Results We found a significant increase in the mutation frequency of NLRP12 rs12460528 co-dominant model, particularly in the dominant model. The dominant model GG + GA of NLRP12 rs12460528 exhibited an OR value of 4.167 (1.847–9.402). Furthermore, the qPCR results a significant upregulation of NLRP12 mRNA expression in psoriatic lesions and peripheral blood. The expression of NLRP12 protein was observed to be significantly elevated in psoriatic tissue by immunohistochemistry and Western Blot. Conclusions The results indicated SNP rs12460528 is a potential locus related to NLRP12 in psoriasis. And allele G had a protective effect on psoriasis. NLRP12 was significantly associated with psoriasis and may play a positively regulating role in psoriasis.