Facile Synthesis of Silver Nanoparticles using Green Tea Leaf extract and Evolution of Antibacterial activity

Abstract

Abstract The scientific society is exploiting the use of nanoparticles in nano-medicine, and biomedical applications. In the field of biomaterial and bio-nanotechnology, silver nanoparticles (AgNPs) are playing an important role due to their potential physical, chemical and biological properties ranging in activities from antibacterial, antiviral, antifungal, and anticancer treatment. Green synthesis technology is one of the most cost-effective, eco-friendly, and biologically safe methods. Green Tea leaf extract can reduce silver to AgNPs and enhance antibacterial activity. In this work, we demonstrate antibacterial activity effect employing green synthesis of AgNPs with green tea leaf extract. The UV-Vis and FTIR results showed confirming the formation of AgNPs and the presence of chemical groups enhancing the antibacterial activity of AgNPs. The synthesized AgNPs with green tea leaf extract were crystalline with a quasi-spherical shape with a diameter from 30 to 150nm. The antibacterial activity of the AgNPs in three different concentrations showed that 120mg/ml sample possesses higher ant-bacterial activity (significantly high killing ability) against E-coli than chemically produced AgNPs. These results confirm a more significant antibacterial effect of the biogenic AgNPs with low cytotoxicity than the AgNPs produced chemically. These findings can be used to treat chronic infections, diseases, and other biomedical applications.