Therapeutic Effect of San-Zi-Yang-Qin Decoction on Obese Pre-hypertension: A Non-targeted Combined with Pseudo-targeted Metabolomics

Abstract

Abstract Background Evaluation of the intervention effect of SZD on high-fat and high-salt induced obese Pre-hypertension rats from the perspective of metabolomics, and to explore the metabolic mechanism of SZD for the treatment of obese Pre-hypertension Methods In this study, the efficacy of SZD was evaluated by blood pressure, body weight, Lee’s index, and biochemical indexes, and the mechanism of SZD in the treatment of obese prehypertensive rats induced by high-salt the high-fat mode was explored through non-targeted metabolomics combined with pseudo-targeted metabolomics. Results SZD intervention reduced systolic blood pressure(SBP), diastolic blood pressure(DBP), mean arterial pressure, and reduced body weight and Lee's index in rats, which had some improvement effect on obesity. It also reduced triglyceride(TG), total cholesterol(TC), Low-density lipoprotein cholesterol(LDL),Hypersensitivity C-reactive protein(Hs-CRP) levels in rats, but had no significant elevation effect on High-density lipoprotein cholesterol(HDL). Analysis of 35 biomarkers in the model and 31 biomarkers in the SZD intervention by non-targeted metabolomics revealed that SZD interfered with 7 of these metabolites (estradiol, sphingosine, TXB2, LysoPC (20:2), LysoPE (22:0), LysoPC (22:5), LysoPC (20:0)). The further content analysis of 7 metabolites by pseudo-targeted metabolomics revealed an increase in estradiol and sphingomyelin,and a decrease in LysoPC (20:2), LysoPE (22:0), LysoPC (22:5), and LysoPC (20:0) after SZD intervention, which involved in glycerophospholipid metabolism, sphingolipid metabolism, linoleic acid metabolism, and arachidic acid metabolism. Conclusions SZD was finally found to improve obesity and decreased blood pressure.The preliminary investigation of the efficacy mechanism of SZD intervention in obese Pre-hypertension enriched the pharmacological effects of SZD.