Stimulation of the Biofilm’s of a marine bacterium, Pseudoalteromonas agarivorans - by Fucoidan extracted from macroalgae

Abstract

Abstract Bacterial biofilm growth in aquatic environments can be promoted by natural compounds, such as polysaccharides and proteins. Fucoidan is a group of marine sulfated polysaccharides found in cell wall matrix of brown and red algae. In the present study, efficiencies of fucoidan extracts obtained by two alternate methods from Cystoseira sp. and Padina pavonica were investigated of stimulation of biofilm development of Pseudoalteromonas agarivorans. The fucoidan rich extracts were tested for adhesion of biofilm bacterium in black polystyrene flat-bottom microplates. In the biofilm experiments, the extracts were found to stimulate biofilm development. The maximum mean for the biofilm stimulation efficiencies were 67.52% for SG1 - Cystoseira sp. and 67.50% for SG1 - P. pavonica. The results highlighted that Pseudoalteromonas agarivorans was able to adhere to the plates, and the growth rates were increased with the addition of fucoidan extracts. This research indicated that fucoidan can be effective in biofilm development strategies in aquatic environments.