Long noncoding RNA RP3-340N1.2 affects the progression of lung adenocarcinoma cell lines by targeting miR-134-5p

Abstract

Abstract Background With the development of molecular biotechnology and related databases, increasing numbers of long non-coding RNAs (lncRNAs) have been proven to play important roles in the occurrence and development of tumors. Results In this study, we identified a novel lncRNA, RP3-340N1.2, which has not been reported as associated with lung adenocarcinoma (LUAD). Our results showed that the expression level of RP3-340N1.2 was upregulated significantly in three LUAD cell lines (A549, NCIH1299, and NCIH1975) and lentivirus-mediated knockdown of RP3-340N1.2 inhibited the differentiation, proliferation, migration, invasion, cell cycle, and other biological processes of tumor cells significantly. Bioinformatic software predicted the downstream target of RP3-340N1.2, which was verified using dual luciferase assays and other methods. We found that RP3-340N1.2 could competitively bind miR-134-5p in LUAD cells, and this microRNA has a significant regulatory effect on the expression of epidermal growth factor receptor (EGFR), which leads to increased migration and invasion of LUAD cells. This discovery provided a possible mechanism by which RP3340N1.2 participates in the development of LUAD. Conclusions In conclusion, our results showed that RP3340N1.2 regulates the expression of EGFR indirectly by targeting miR-134-5p, thereby promoting the progression of LUAD. Therefore, RP3-340N1.2 might become a valuable biomarker and therapeutic target in LUAD.