Affiliation:
1. Tamagawa University College of Agriculture Graduate School of Agriculture: Tamagawa Daigaku Nogakubu Daigakuin Nogaku Kenkyuka
2. Nagasaki Agriculture and Forestry Technical Development Centre Fruit Tree and Tea Research Unit
3. Nagasaki Agriculture and Forestry Development Centre Fruit Tree and Tea Reseach Unit
4. Tamagawa University College of Agriculture
5. Tamagawa University
Abstract
Abstract
Colletotrichum is the second most common pathogen that causes loquat fruit rot in Nagasaki prefecture, Japan. To clarify the anthracnose disease cycle in loquat trees, pathogens were isolated from collected asymptomatic flowers and rotten fruits. The isolates were identified at the species level based on morphological and molecular phylogenetic analyses. Nineteen isolates were obtained from 148 collected rotten fruits. The isolates were identified as C. fioriniae (12 isolates), C. nymphaeae (2 isolates), C. gloeosporioides (1 isolate), and four unidentified species. From 900 asymptomatic flowers, C. fioriniae (5 isolates), C. gloeosporioides (1 isolate), C. siamense (2 isolates), an unknown species (11 isolates) belonging to the acutatum species complex, and 2 were unidentified. All isolates exhibited pathogenic reactions to leaves and fruits. These results imply that C. fioriniae and C. gloeosporioides infect flowers and cause loquat fruit rot after maturation. However, those in other species remain unclear. We were unable to prove the reason for the differences in the composition of species in fruits and flowers and their proportions in this study. An isolate obtained from asymptomatic flowers that also cause anthracnose disease in loquat was identified as a novel species of Colletotrichum, and was named C. nagasakiense sp. nov.
Publisher
Research Square Platform LLC
Reference30 articles.
1. Mango anthracnose: economic impact and current options for integrated managaement;Arauz LF;Plant Dis,2000
2. Colletotrichum acutatum can establish on sweet and sour cherry trees throughout the growing season;Børve J;Eur J Hortic Sci,2013
3. Colletotrichum - current status and future directions;Cannon PF;Stud Mycol,2012
4. A method for designing primer sets for speciation studies in filamentous ascomycetes;Carbone I;Mycologia,1999
5. Calonectria species and their Cylindrocladium anamorphs: species with sphaeropedunculate vesicles;Crous PW;Stud Mycol,2004