Identification of key genes related to immune infiltration in cirrhosis via bioinformatics analysis

Abstract

Abstract Background:Cirrhosis is the most common subclass of liver disease worldwide and correlated to immune infiltration. However, the immune-related molecular mechanism underlying cirrhosis remains obscure.Methods:Two gene expression profiles GSE89377 and GSE139602 were investigated to identify differentially expressed genes (DEGs) related to cirrhosis. Enrichment analysis for DEGs was conducted. Next, the immune infiltration of DEGs was evaluated using CIBERSORT algorithm. The hub DEGs with tight connectivity were identified using the String and Cytoscape databases, and the expression difference of these hub genes between normal liver and cirrhosis samples was determined. Finally, the association between hub DEGs and immune cell infiltration was explored by Spearman method.Results: Among the 299 DEGs attained, 136 were up-regulated and 163 were down-regulated. Then the enrichment function analysis of DEGs and CIBERSORT algorithm showed significant enrichment in immune and inflammatory responses. And four hub DEGs (ACTB, TAGLN, VIM, SOX9) were identified. Finally, the immune infiltration analysis indicated that, these hub DEGs were highly related to immune cells. Conclusions:This study revealed key DEGs involved in inflammatory immune responses of cirrhosis, which could be used as biomarkers or therapeutic targets of cirrhosis.