Dual-Modality Imaging for Monitoring Thrombosis via Platelet GPIIb/IIIa Receptor Targeted cyclic RGDfK Microbubbles

Abstract

Abstract Backgound: Acute thrombotic events play a vital role in cardiovascular diseases. Our study aims to investigate an ultrasound microbubble fordual-modality imaging of thrombi. Methods:DID-DSPE-PEG-cRGD-Microbubbles wereprepared by mechanical vibration and Chemical chelation methods. In vivofluorecence and ultrasound imaging were performed after by intravenous injection of DID-DSPE-PEG-cRGD-Microbubbles respectively. Results: This microbubbles exhibitedgood binding affinity to activated platelets with significant fluorescence and ultrasonic signals. Ultrasound and fluorescence imaging revealed that the microbubbles accumulated at the site of the thrombus in the carotid artery, and immunofluorescence showed that microbubbles could be detected in the carotid artery thrombus. Conclusion: We successfully synthesized novel microbubble cRGD-MBs, which indicateexcellent potential for immediate diagnosis of acute thrombus in vivo.