The recycling substitute of pilosula velutipes in the growth medium: from the perspective of microbiomics

Abstract

Abstract Using waste cotton as substrate for straw mushroom production has great nutritional support value and commercial value. The production of Volvariella volvacea depends on the physicochemical and microbial properties of the substrate. Due to the strong influence of microorganisms and the complexity of substrate culture, Volvariella volvacea production was finally induced under controlled environmental conditions. The interaction of microorganisms in the process of substrate fermentation has greatly affected the production and colonization of Volvariella volvacea. In this study, the characteristics and production performance of bacterial microbiota in Volvariella volvacea substrate (fermentation period) were compared, and the microbial community in Volvariella volvacea substrate was identified. The growth of Volvariella volvacea changed the microbial composition and structure of substrate. 16S rRNA was used to study the microbial diversity of Volvariella volvacea culture material at different stages and its effect on substrate.The results showed that: in the whole fermentation cycle, the total number of bacterial species in the substrate was negatively correlated with time, and the abundance of Proteus in the substrate was high (accounting for 40%), which decreased first and then increased with the fermentation time. In all samples, at the species level, the relative abundance of amino acid metabolism and sugar metabolism was high, which reflected that the substrate played a great role in nourishing Volvariella volvacea.The technology used in this study provides the overall and detailed characteristics of microbial composition changes in Volvariella volvacea substrate. Analyze the influence of substrate microorganisms on Volvariella volvacea culture, determine the most suitable substrate environment, improve resource utilization, understand the optimal fermentation conditions corresponding to substrate formula, effectively use different substrates in different regions as substrates for Volvariella volvacea culture, and reduce the transportation cost of raw materials. Improve the profits of edible fungi industry. The knowledge generated will help to improve matrix formulation (selection of base materials) and accelerate matrix production, for example, through biotechnology interventions in the form of customized biostimulants and the design of environmentally sustainable bio based materials.