miR-224-5p inhibits proliferation and invasion of non-small cell lung cancer by targeting IL6ST inactivation of JAK2/STAT3 signal pathway

Abstract

Abstract Non-small cell lung cancer (NSCLC) accounts for more than 85% of lung cancer, with high morbidity and mortality. Studies have shown that microRNA can specifically inhibit the progression of NSCLC. MiR-224-5p can regulate tumor progression in many cancers, but its function and mechanism in NSCLC aren’t clear. In this study, we found that the expression of miR-224-5p was reduced in NSCLC tissue and cells than normal lung tissue and cells. At the same time, miR-224-5p negatively regulates the proliferation and migration of NSCLC cells. Inhibition of miR-224-5p expression in A549 cells could promote cell proliferation, invasion, migration and VM formation in vitro and tumor growth and lung metastasis in vivo, while over expression of miR-224-5p in H226 cells reversed the effect. Besides, we predicted target gene and found that IL6ST is a potential target gene of miR-224-5p. The expression of miR-224-5p is negatively correlated with IL6ST and activation of JAK2/STAT3 pathway. Over expression of IL6ST reversed the effects of miR-224-5p on migration, invasion and activation of JAK2/STAT3 pathway in H226 cells. In conclusion, this study revealed that miR-224-5p can inhibit the proliferation and invasion of NSCLC by targeting inhibition of IL6ST gene transcriptional and inactivation of JAK2/STAT3 signal pathway.