Molecular characterisation of human JC polyomavirus and risk of developing prostate adenocarcinoma at an early age.

Abstract

Abstract Background: The putative functions of JC polyomavirus (JC PyV) infections and Simian Virus 40 (SV40) in the initiation of prostate tumors have recently been discovered. Objective: The objective of our study is to determine the frequency and the genotype of JCPyV and SV40 infections and the clinical correlation of tumor characteristics. Material and methods: 80 samples were analyzed 50 prostate cancer patients and 30 control cases. DNA extracts were obtained from 50 men treated at the University Hospital of the city of Rabat (Morocco) between June 2021 and February 2022. These samples were then genotyped and analyzed using Sanger. Results: Ten JC PyV infections were detected, and 50% of patients had a Gleason score of 6. A significant association between the following variables was noted: age at diagnosis and JC PyV infection (P=0.004); background medical (P=0.034); alcohol (P=0.035); BMI (P<0.001). However, no significant differences were observed between men infected and uninfected concerning other settings pathological, including pathological Gleason score, antigen prostate-specific (PSA), history surgical, stage histology of the tumor (P =0.172, P=0.107, P=1.00, P=0.258, and P=0.884, respectively). So, we don't have could detect SV40 infection in PCa samples, suggesting this virus is unrelated to the development of this type of tumor. The evolution structure of the JC PyV according to the sequences previously aligned was discussed. Conclusion: Our results reveal the correlations between the parameters of the Clinico-pathology of prostate tumors and JC PyV infections. Research prospects with a wide size sample are essential to elucidate statistically the relationship between viral infections and parameters of prostate tumors.