A unique microbiota for normal breast tissue

Author:

German Rana1,Marino Natascia2,Hemmerich Chris3,Podicheti Ram3,Rusch Douglas B.3,Stiemsma Leah T.4,Gao Hongyu5,Xuei Xiaoling5,Rockey Pam1,Storniolo Anna Maria2

Affiliation:

1. Susan G. Komen Tissue Bank at the IU Simon Comprehensive Cancer Center, Indianapolis, IN 46202

2. Department of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202

3. Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405

4. Pepperdine University, Natural Science Division, Malibu, CA, 90263

5. Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202

Abstract

Abstract Background Microbial dysbiosis has emerged as a factor in most cancers, including breast cancer. However, whether changes in microbial composition of the breast are a cause, or a consequence, of tumor development remains unclear. Here, we analyzed the microbiota of normal breast tissue (N = 403) by sequencing the nine hypervariable regions of the 16S rRNA gene (V1V2, V2V3, V4V5, V5V7, and V7V9). Results We defined the V1V2 amplicon as optimal for our analysis and identified Lactobacillaceae (Firmicutes phylum), Acetobacterraceae, and Xanthomonadaceae (both Proteobacteria phylum) as the most abundant families in the normal breast. However, Ralstonia (Proteobacteria phylum) was more abundant in breast tumors and histologically normal tissues adjacent to malignant tumors (N = 31 and N = 61, respectively). We also conducted a correlation analysis between the microbiome and known breast cancer risk factors. Abundances of the bacterial taxa Acetotobacter aceti, Lactobacillus vini, Lactobacillus paracasei, and Xanthonomas sp. were associated with age (p < 0.0001), racial background (p < 0.0001), and parity (p < 0.0001). Finally, transcriptome analysis of normal breast tissues showed an enrichment in metabolism- and immune-related genes in the tissues with abundant Acetotobacter aceti, Lactobacillus vini, Lactobacillus paracasei, and Xanthonomas sp., whereas the presence of Ralstonia in the normal tissue was linked to genes involved in the carbohydrate metabolic pathway. Conclusions This study defines the microbial features of normal breast tissue, thus providing a basis to understand cancer-related dysbiosis. Moreover, the findings reveal that lifestyle factors can significantly affect the breast microbial composition.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3