Genetic characteristics of a large pediatric cohort of patients with inborn errors of immunity: single-center experience

Author:

Kuzmenko Natalia1,Alexenko Maxim1,Mukhina Anna1,Rodina Yulia1,Fadeeva Mariia1,Pershin Dmitrii1,Kieva Amina1,Raykina Elena1,Maschan Miсhael1,Novichkova Galina1,Shcherbina Anna1

Affiliation:

1. Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology

Abstract

Abstract

More than 450 genetic defects result in inborn errors of immunity (IEI). Their individual prevalence in specific cohorts is influenced by national characteristics and other factors. We present results of genetic testing conducted in 1809 Russian children with IEI. Genetic defects confirming IEI were found in 1112 out of 1809 (61.5%) probands. These defects included variants in 118 single genes (87.9% of patients) and aberrations in 6 chromosomes (11.8%). Notably, three patients harbored pathogenic variants in more than one IEI gene. Large deletions constituted 5% of all defects. Out of the 799 original variants, 350 (44%) have not been described previously. Rare genetic defects (10 or fewer patients per gene) were identified in 20% of the patients. Among 967 probands with germline variants, defects were inherited in an autosomal dominant manner in 29%, X-linked in 34%, and autosomal recessive in 37%. Four females with non-random X-inactivation exhibited symptoms of X-linked diseases (BTK, WAS, CYBB, IKBKG gene defects). Despite a relatively low rate of consanguinity in Russia, about 50% of autosomal recessive gene defects were found in a homozygous state. Notably, 28% of these cases carried “Slavic" mutation of the NBN gene or known hot-spot mutations in other genes. The diversity of IEI genetic forms and the high frequency of newly described variants underscore the genetic heterogeneity within the Russian IEI group. The new variants identified in this extensive cohort will enriching genetic databases.

Publisher

Springer Science and Business Media LLC

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