Promoting the Proliferation of Osteoarthritis Chondrocytes by Resolvin D1 Regulating NLRP3/caspase-1 Signaling Pathway

Abstract

AbstractBackgroundResolvin D1 could reduce the inflammatory, catabolic response of OA chondrocyte, and promote the repair of various tissues.Our goal was to explore whether RvD1 could inhibit NLRP3/caspase-1 signaling pathway, slow down the occurrence of pyroptosis of OA chondrocytes, and then promote the proliferation of OA chondrocytes and repair of articular cartilage.MethodsAnimal care and use protocols comply with the National Institutes of Health Guide for the Care and Use of Laboratory Animals.After the rat osteoarthritis model was established, RvD1 was injected and a control group trial was set up, Joint specimens were then collected.The micro-CT system was used to determine the acquisition of a 3D model of the rat knee joint. Chondrocytes were stained with toluidine blue, Then the cover slip was examined under a light microscope. EDU was used to detect the proliferation of chondrocytes.Western Blot,RT-qPCR and Immunofluorescence was used to detect markers in experiments.ResultsCompared with the control group, RvD1 can promote OA chondrocytes multiplication and inhibit chondrocytes pyrosis by regulating the cell cycle.Belnacasan is a specific inhibitor of caspase-1, Treatment of OA chondrocytes with Belnacasan and RvD1 showed that Belnacasan could specifically inhibit the conduction of pyroptosis pathway induced by caspase-1, and the synergistic inhibitory effect with RvD1 was more significant.ConclusionRvD1 promotes the proliferation of OA chondrocytes by inhibiting the expression of caspase-1 to regulate NLRP3/caspase-1 signaling − 8 pathway. At the same time, RvD1 promoted the repair of articular cartilage and retarded the progression of OA in rats.