Molecular detection and analysis of a Enzootic Nasal Tumor Virus 2 in china

Abstract

Abstract Enzootic nasal tumor virus (ENTV) is a Betaretrovirus, which is associated with the neoplastic transformation of epithelial cells of the ethmoid turbinates. It has two types, ENTV-1 in sheep and ENTV-2 in goats, respectively. As we know, the incidence rate of ENTV-2 related diseases in China and even the world has increased in recent years. In this study, an infection of ENTV-2 was detected by RT-PCR in a goat flock in Chongqing City, and a full-length genome of ENTV-2 (named ENTV-2-CQ) with 7469 nucleotides (nt) was sequenced. Comparing with full-length genome sequences revealed that ENTV-2-CQ strain has high identity to the reference complete genomes (86.63–99.41%) at the nucleotide level. ENTV-2-CQ shared the highest nucleotide identity with a ENTV-2FJ isolate (GenBank accession number MK559457.1). Phylogenetic analysis shows that ENTV-2-CQ and ENTV-2 are located in the same major branch, mainly related to isolates from China from 2015 to 2022. Our research will contribute to a deeper understanding of the epidemiology and pathogenesis of this important retrovirus.