miR-122 dysregulation is associated to the type2 diabetes mellitus-induced dyslipidemia and hyperglycemia independently to its variant rs17669

Abstract

Abstract Background: miR-122 is a liver specific micro RNA that participates in the regulation of carbohydrate and lipid metabolism. rs17669 variant positioned at the flanking region of miR-122 and may be affects its stability and maturation. Therefore, this study was aimed to investigates the association between rs17669 polymorphism with the miR-122 circulating level, risk of type 2 diabetes mellitus (T2DM) development and biochemical parameters in these patients and matched healthy controls. Methods and Results: Two hundred and ninety-five subjects (controls; n=145 and T2DM; n=150) enrolled in this study. rs17669 variant genotyping was done by ARMS-PCR. Serum biochemical parameters including lipid profile and glucose were measured by colorimetric kits. Insulin and Glycated hemoglobin (HbA1c) were assayed by using capillary electrophoresis and ELISA methods respectively. miR-122 expression was measured by real-time PCR. There was no significant difference in allele and genotype distribution between study groups (P>0.05). rs17669 variant has not any significant association with miR-122 gene expression and biochemical parameters (P>0.05). miR-122 expression level in T2DM patients was considerably higher than that in control subjects (5.7±2.4 vs 1.4±0.78) (P>0.05). Furthermore, there were a positive and significant correlation between miR-122 fold change with LDL-C, sdLDL, FBS and insulin resistance (P>0.05). Conclusions: It can be concluded that miR-122 rs17669 variant is not associated to the miR-122 expression and T2DM-associated serum parameters. Furthermore, it can be suggesting that miR-122 dysregulation involved in T2DM development through the inducing dyslipidemia, hyperglycemia as well as resistance to insulin.