Extracellular vesicle-associated microRNA signatures related to vascular invasion in early-stage lung adenocarcinoma

Abstract

Abstract Vascular invasion (VI) is a fundamental step toward the spread of cancer. Extracellular vesicles (EVs) promote cellular communication by shuttling cargo, such as microRNAs (miRNAs). However, whether EV-associated miRNAs serve as biomarkers for VI remains unclear. This study aimed to identify EV-associated miRNAs related to VI and validate the miRNA levels from patients with early-stage lung adenocarcinoma (LADC). Blood samples were collected from patients undergoing pulmonary resection for stage I LADC before surgery. The patients were classified into three groups according to the presence of VI and postoperative recurrence. Serum-derived EVs in the derivation cohort were used for small RNA sequencing, while the selected VI miRNA candidates were validated via real-time quantitative polymerase chain reaction using 44 patient and 16 healthy donor samples as the validation cohorts. Five miRNAs (miR-99b-3p, miR-26a-5p, miR-93-5p, miR-30d-5p, and miR-365b-3p) were assessed, and miR-30d-5p (p = 0.036) levels were significantly downregulated in the VI-positive group. MiR-30d-5p levels in healthy donors were lower than those in LADC patients. Patients with high miR-30d-5p levels had favorable survival compared to those with low miR-30d-5p levels. MiR-30d-5p level in EVs may serve as a promising biomarker for detecting VI in patients with early-stage LADC.