Development of genogroup-specific ELISA based on the VP1 protein to detect antibodies to GIV and GVI feline norovirus-Reference-Cited by-同舟云学术

Development of genogroup-specific ELISA based on the VP1 protein to detect antibodies to GIV and GVI feline norovirus

Published:2024-07-11 Issue: Volume: Page:
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Author:

Takano Tomomi¹^ORCID,Tsuhama Hideyuki¹,Matsumoto Yuka¹,Doki Tomoyoshi¹,Kusuhara Hajime²

Affiliation:

1. Kitasato University School of Veterinary Medicine Graduate School of Veterinary Medicine: Kitasato Daigaku Juigakubu Daigakuin Juigakukei Kenkyuka

2. Health and Environment Research Institute

Abstract

Feline norovirus (FNoV) is a potential pathogen of feline gastroenteritis and has two gene groups (GVI and GVI). Fewer epidemiological studies have been conducted on FNoV. We designed two ELISAs to identify genogroup-specific FNoV antibodies for serological surveillance. Analysis of sera from cats experimentally infected with GIV/GVI FNoV and SPF cats confirmed that the two recombinant proteins react in a genogroup specific manner. Among 183 samples, GIV FNoV and GVI FNoV antibody-positive rates were 6.6 and 26.2%, respectively. The FNoV antibodies of both genogroups were detected in sera collected in 2005, seven years before the presence of FNoV was reported.

Publisher

Springer Science and Business Media LLC

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