Affiliation:
1. University of Alberta
2. University of Alberta Faculty of Medicine & Dentistry
3. University of Alberta College of Social Sciences and Humanities
4. UBC: The University of British Columbia
5. Schulich School of Medicine and Dentistry: Western University Schulich School of Medicine & Dentistry
Abstract
Abstract
Background
Proteinase-activated receptor 2 (PAR2) is a G-protein coupled receptor associated with many inflammatory diseases, including asthma. We have shown an association between PAR2 expression in peripheral blood monocytes and asthma severity as well as blood PAR2 mRNA level and lung function. Since F2RL1 (the gene encoding PAR2) polymorphisms affect PAR2 expression, we hypothesize they may affect asthma severity.
Methods
We recruited 76 subjects with asthma of varying severity and collected clinical (FEV1 [% predicted], FEV1/FVC, IgE) and immunological (PAR-2 mRNA, blood eosinophils) disease parameters. We also genotyped these individuals for 3 F2RL1 SNPs (-45C/T, -149C/G, c.621C/T).
Results
We found that the F2RL1 SNP “C” allele of -45C/T (rs1529505) was associated with PAR-2 mRNA and blood eosinophils. F2RL1 SNP c.621C/T (rs631465) was associated with PAR-2 mRNA. The F2RL1 SNP -149C/G (rs2242991) had no association with any of the parameters studied. This study identified one F2RL1 SNP rs1529505 is associated with parameters of asthma, but not asthma severity.
Conclusion
Larger studies are needed to further elucidate the role of PAR2 in the pathophysiology of asthma and the influence of genetic variation.
Publisher
Research Square Platform LLC