Utilization of the ethyl acetate fraction of Padina boergesenii as a natural UV filter in sunscreen cream formulation

Abstract

Abstract Brown seaweeds due to their wide range of bioactive compounds have a high ability to inhibit free radicals and protect against ultraviolet rays. In the present study, the ethyl acetate fraction was isolated from the Padina boergesenii brown alga. Antioxidant activity (by inhibition DPPH free radical method) and cytotoxicity against UVB-induced cytotoxicity in HaCaT human keratinocytes were evaluated. Then, this fraction was used as a biofilter in the formulation of sunscreen and was investigated physical properties and stability. The results showed that the ethyl acetate fraction could inhibit DPPH radical scavenging (54 ±1 %) and the ability to cell viability of HaCaT keratinocytes exposed to UVB irradiation (81.2 ± 0.1 %). The results of the stability study of the cream formulated with ethyl acetate fraction showed that temperatures 4°C and 25°C have high stability and at 40°C on the 28th day, was observed a slight decrease in its stability. The pH and SPF of the cream formulated with ethyl acetate fraction were reported at 5.8 and 20.55, respectively. Also, the DPPH scavenging activity of the cream was not altered for 28 days of storage at temperatures of 4 - 40°C. According to the obtained results, it was proved that the sunscreen formulated with ethyl acetate fraction of Padina boergesenii brown seaweed has promising properties and characteristics that can create a new opportunity for the development of cosmetics and skin care products.