Affiliation:
1. International Centre for Diarrhoeal Disease Research
2. Johns Hopkins Bloomberg School of Public Health
3. Tufts University
Abstract
Abstract
Background
Despite the advancement in our understanding of cholera and its etiological agent, V. cholerae, the prevention and treatment of the disease are often hindered due to rapid changes in drug response pattern, serotype, and the major genomic islands namely, the cholera toxin phage, and related genetic characteristics. In the present study, V. cholerae (n = 172) associated with endemic cholera in Dhaka during the years 2015–2021 were analyzed for major phenotypic and genetic characteristics, including drug resistance patterns.
Results
Results revealed that the V. cholerae strains belonged to serogroup O1 biotype El Tor carrying El Tor -specific genes rtxC, tcpA El Tor, and hlyA El Tor, but possessed classical-biotype cholera toxin. Serotypes of V. cholerae strains differed temporally in predominance with Inaba during 2015–2017, and again in 2020–2021, while Ogawa was the predominant serotype in 2018–2019. Also, ctxB1 was predominant in V. cholerae associated with cholera during 2015–2017, while ctxB7 was predominant in 2018, and in the subsequent years, as observed until 2021. V. cholerae strains differed in their antibiotic resistance pattern with a majority (97%) being multi-drug resistant (MDR) and belonging to eight sub-groups. Notably, one of these MDR strains was resistant to eleven of the eighteen antibiotics tested, with resistance to fourth-generation cephalosporin (cefepime), and aztreonam. This extreme drug resistant (XDR) strain carried resistance-related genes namely, extended-spectrum β-lactamases (ESBL), blaOXA-1 and blaPER-3.
Conclusion
The observed temporal switching of serotypes, as well as the ctxB genotype, and the emergence of MDR/XDR V. cholerae and their association with endemic cholera in Dhaka underscore the need for routine monitoring of the pathogen for proper patient management.
Publisher
Research Square Platform LLC
Reference41 articles.
1. Kaper JB, Morris JG, Levine MM. Cholera. 1995;8(1):48–86.
2. Evidence for several waves of global transmission in the seventh cholera pandemic;Mutreja A;Nature. 2011 Sep
3. Sack DA, Sack RB, Nair GB, Siddique AK. Cholera. Lancet. 2004 Jan 17;363(9404):223–33.
4. Evolution of new variants of Vibrio cholerae O1;Safa A;Trends Microbiol. 2010 Jan
5. Vibrio cholerae O1 with reduced susceptibility to ciprofloxacin and azithromycin isolated from a rural coastal area of Bangladesh;Rashed SM;Front Microbiol,2017
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