Isolation and characterization of forty-two polymorphic simple sequence repeat markers from Mastacembelus armatus

Author:

Luo Laifu1,Chen Chen2,Ji Liqin2,Wu Congcong2,Li Wei2,Liu Xiaoli2,Hong Xiaoyou2,Zhu Xinping2,Zhang Jiping1

Affiliation:

1. Foshan University

2. Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences

Abstract

Abstract A total of 42 polymorphic simple sequence repeat (SSR) markers have been developed and characterized from genome sequences of Mastacembelus armatus to safeguard its natural resources. Polymorphisms of these SSR markers were evaluated in a natural population of 35 individuals. The number of alleles per locus ranged from two to fourteen, observed heterozygosity and expected heterozygosity varied from 0.031 to 0.813, and from 0.091 to 0.852, respectively. Three SSR loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These loci are the first time to be characterized and will be useful for genetic conservation and marker-assisted breeding for M. armatus.

Publisher

Research Square Platform LLC

Reference4 articles.

1. Population genetics of zig-zag eel (Mastacembelus armatus) uncover gene flow between an isolated island and the mainland China [J];Yang Y;Front Mar Sci,2023

2. Shi Qiong F, Mingjun Z Yong (2015) Economic fish record of China [M]. Huazhong University of Science and Technology Press

3. An economic method for the fluorescent labeling of PCR fragments [J];Schuelke M;Nat Biotechnol,2000

4. A retrospective assessment of the accuracy of the paternity inference program CERVUS [J];Slate J;Mol Ecol,2000

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