Study on Interaction and Binding Properties of Terpenes repellent active compounds with Odorant Binding Protein 9B in Tribolium castaneum

Author:

Sang Yu-Li1,Qiang Xin-Yue1,Dai Lu1,Chen Li-Jiang1,Liu Yu-Feng1,Hao Yan-Jun2,Liu Jing-Yu3,Qi Jian-Zhong4

Affiliation:

1. Liaoning University

2. Liaoning University of Traditional Chinese Medicine

3. Wuxi STA Pharmaceutical Co., Ltd.

4. China Resources Chenzhou Pharmaceutical Co., Ltd.

Abstract

Abstract To screen the odorant binding proteins (OBPs) of Tribolium castaneum related to repellent activity and explore the repellent mechanism of terpenoids by studying the interaction between ligands and TcOBPs. Behavior tests indicated that (1R)-(+)-α-Pinene and β-Caryophyllene had repellent activity against T. castaneum. Through homology modeling and molecular docking, we screened TcOBP-9B, which is highly expressed in antennae and has strong interactions with (1R)-(+)-α-Pinene, β-Caryophylne, as target protein for recombinantly expressed. Fluorescence spectra and UV absorption spectra showed that TcOBP-9B had strong bind to (1R)-(+)-α-Pinene and β-Caryophyllene with concentration-dependent in static quenching. Thermodynamic date revealed that they formed stable complexes through van der Waals force, electrostatic and hydrophobic interactions. By fluorescence competitive binding assay, molecular docking and circular dichroism spectra, the binding sites of TcOBP-9B was confirmed as the α-helix, where the ligands were competitively bound to 1-NPN. These results suggested that TcOBP-9B was a significant target protein associated with repellent activity, providing new approach for screening repellents against T. castaneum.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3