Affiliation:
1. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
2. NEOMICS Institute
3. Hunan Research Institute for Nonferrous Metals Co., Ltd.
4. Central South University
Abstract
Abstract
Deciphering the impact of single and combined contamination of total petroleum hydrocarbons (TPH) and heavy metals on soil microecosystems is essential for the remediation of contaminated habitats, yet it remains incompletely understood. In this study, we employed high-throughput sequencing to investigate the impact of single TPH contamination, single metal contamination, and their co-contamination on soil microbial diversity, assembly mechanisms, composition, ecological function, and resistome. Our results revealed that contamination led to a reduction in alpha diversity, with single contamination displaying lower diversity compared to co-contamination, depending on the concentration of pollutants. Community beta diversity was primarily driven by turnover rather than nestedness, and narrower ecological niches were detected under pollution conditions. The neutral community model suggested that homogenizing dispersal played a significant role in the community assembly process under single TPH or co-contamination, while homogeneous selection dominated under heavy metals pollution. Procrustes analysis demonstrated a correlation between community composition and functional divergence, while Mantel tests linked this divergence to concentrations of Cr, Cr6+, Pb, and TPH. Interestingly, soils co-polluted with TPH and heavy metals exhibited similar genera, community functions, and resistomes as soils contaminated with only metals, highlighting the significant impact of heavy metals. Ecological functions related to carbon (C), nitrogen (N), and sulfur (S) cycles were enhanced under TPH pollution but impaired under heavy metals stress. These findings enhance our understanding of soil microecosystems subjected to TPH, heavy metals, and their co-contamination, and carry significant implications for environmental microecology and pollutant risk assessment.
Publisher
Research Square Platform LLC
Reference61 articles.
1. Soil contamination, risk assessment and remediation;Ashraf MA;Environmental risk assessment of soil contamination,2014
2. Chu D: Effects of heavy metals on soil microbial community. In: 2018 2018: IOP Publishing; 2018: 012009.
3. Effect of petroleum products on soil catalase and dehydrogenase activities;Achuba FI;Open Journal of Soil Science,2014
4. Core Flood study for enhanced oil recovery through ex-situ bioaugmentation with thermo- and halo-tolerant rhamnolipid produced by Pseudomonas aeruginosa NCIM 5514;Varjani SJ;Bioresource technology,2016
5. Acinetobacter sp. as the key player in diesel oil degrading community exposed to PAHs and heavy metals;Czarny J;Journal of hazardous materials,2020