Biogenic potentiality of cationic peptides isolated from mangrove Streptomyces albogriseolus

Abstract

Abstract Streptomyces albogriseolus from mangrove soil was isolated from the coastal region of Mangalore and had morphologically different aerial and substrate mycelium as well as diffusible coloration. The cylindrical sporiangiophore of Streptomyces albogriseolus was visible in the scanning electron microscope image together with tightly packed open spiral filaments. Streptomyces albogriseolus was grown in ISP2 media to test the intracellular peptides' potential for antibacterial action. The collected biomass was pH 7.2 sodium phosphate buffer homogenized. Streptomyces albogriseolus's peptides demonstrated notable anti-oxidant, anti-inflammatory, and anti-biofilm capabilities. The intracellular peptides were subjected to ion exchange chromatography separation using CM-cellulose column after the antibacterial activity was confirmed. Using BSA as a reference, Lowry's technique was used to estimate the peptide fraction. Based on reverse phase HPLC and LCMS analysis of peptides with molecular masses of 28.61 kDa, the CM cellulose pk 5 fraction was further divided. The ability of a peptide to permeate membranes was evaluated in an aliquot with an estimated concentration of 3.75g in 10µl. By using a field-emission scanning electron microscope, it was discovered that the Streptomyces albogriseolus peptide had a membrane permeabilization effect on Klebsiella pneumoniae, causing pore formation and membrane disintegration. Acridine orange/ethidium bromide labelling and flow cytometric measurement for PI cell uptake were used to further demonstrate this influence on membrane activity. By submitting the N-terminal amino acid sequence to the SWISS-Prot database and using the taxonomy of the 28.61 kDa peptide from Streptomyces albogriseolus, which belongs to the family "Imidazoleglycerol-phosphate dehydratase," to determine the structure of the peptide complex. Additional research was done to examine the anticancer properties of the Streptomyces albogriseolus peptide, and it was shown that MCF-7 breast cancer cells significantly displayed cytotoxicity and DNA damage.