Synergistic interaction of Artemisia cina n-hexanic extract and Tagetes lucida ethyl acetate extract on Haemonchus contortus

Abstract

Abstract The present study aimed to analyze the possible synergistic activity among the active extracts from Artemisia cina and Tagetes lucida combinations on the Haemonchus contortus nematode parasite of sheep. The work was carried out in vitro on eggs and infective larvae 3 of H. contortus. Also, the characterization of the extracts was made with HPLC. The results were analyzed with SAS 9.1 apply the anova and Tukey test, and the lethal concentration (LC) LC50 and LC90 were determined with regression analysis employed Proc Probit of SAS 9.1. Additionally, the FT was calculated with LC50 and LC90 to determine the synergistic effect. The results demonstrated high efficacy of the two plants studied on both nematode stages egg and larvae L3, as well as of their combinations in where the best inhibition hatch egg was obtained with 50/50% combination of each plant and the best larvae mortality were obtained with 25% A. cina and 75% T. lucida at 10 mg/ml, additionally this combination showed synergistic effect. In conclusion the two plants are an option as anthelmintic non pharmacological due to high bioactive effect and due to their synergistical response.