GREB1L overexpression associates with good clinical outcomes in breast cancer

Abstract

Abstract Background Breast cancer is a malignant tumor with the highest incidence among women in the world. GREB1L is a protein coding gene. Previous studies have shown that GREB1L played an important role in lung adenocarcinoma and gastric adenocarcinoma. Currently, there is no relevant report about its role in breast cancer. Methods The Cancer Genome Atlas database was used to compare the expression level of GREB1L; TISIDB website was used for prognosis analysis; LinkedOmics database was used to predict the potential biological mechanism of GREB1L in breast cancer; Immunohistochemistry was used to detect the GREB1L expression level in breast tissue; Western blot was used to detect the GREB1L expression level in cell lines; Transwell assays, CCK8 cell proliferation assays and clone formation assays were used to detect the migration, invasion and proliferation and clone formation abilities of cells. Results GREB1L was highly expressed in breast cancer tissues and breast cancer cells; KEGG enrichment analysis suggested that GREB1L might participate in the regulation of Hedgehog signaling pathway; GREB1L affected the migration and invasion abilities of MCF7 and MDA-MB-231 cells, but not affected their proliferation and clone formation abilities. The overexpression of GREB1L in breast cancer predicted a favorable prognosis. Conclusion These results showed that GREB1L was involved in the development of breast cancer, and it may be a potential molecular marker to predict the prognosis of breast cancer.