Abstract
Hazelnut is one of the most important nut plants recently suggested as a sustainable source of Taxol. Taxolone is one of the most commonly prescribed chemotherapy drugs for cancer and is widely used for various types of cancer. In the present study, the effect of the phenylalanine concentration (5, 7.5 and 12.5 mg. L− 1) and ultrasonic waves (for 30, 45, 60 and 90 s) were investigated for their effects on cell induction and growth and Taxol and other secondary metabolite production in hazelnut cell cultures. This study used a completely randomized experimental design. The results showed that enhancing the UT for up to 45 s led to an increase in both fresh matter (Fm) and dry matter (Dm) to a certain extent, after which a decreasing trend was observed. An examination of the effects of the treatments on the accumulation of secondary metabolites revealed that phenylalanine and UT had positive effects on these indices, with the highest TFC (1.95 mg. g− 1) was observed in samples grown at 12.5 mg.L1. Moreover, our results showed that UT had a positive impact on the production of Taxol in Hazelnut cell suspensions. Specifically, UT at 45 s had the greatest amount of Taxol (208.41 ppm) in the Hazelnut cell suspension.