Non-targeted metabolomic analysis of highland barley original powder and enzymatic hydrolysis powder (pullulanase combined with α-glucosidase)

Abstract

Abstract Highland barley was used as raw material to prepare slow-digested starch by double-enzyme enzymatic hydrolysis process. The non-targeted metabolomics method was used to analyze the correlation between the differences in metabolites of small molecules before and after double-enzyme enzymatic hydrolysis, and to explore the effect on the metabolism of highland barley starch after double-enzyme enzymatic hydrolysis. UHPLC-QTOF-MS detection showed that the main up-regulated metabolites and metabolic pathways in enzymolysis group were mainly flavonoids, and the main down-regulated metabolites and metabolic pathways were mainly lipid metabolism. The results showed that compared with the control group, the enzymatic hydrolysis group regulated the content of sugars and flavonoids in the enzymatic hydrolysis starch by affecting the metabolic pathways such as glycolysis and flavonoid production, thereby affecting the starch characteristics of the double enzyme enzymatic hydrolysis of highland barley flour and making it produce a more gentle blood glucose response in the human body.