A customizable multiplex protein microarray for antibody testing and its application for tickborne and other infectious diseases

Author:

Krishnamurthy Hari1ORCID,Jayaraman Vasanth1,Krishna Karthik1,Wang Tianhao2,Bei Kang1,Suresh Chithra2,Matilda Shiny2,Rai Alex3,Welc-Falęciak Renata4,Pawełczyk Agnieszka5,Blanton Lucas6,Chrdle  Aleš7,Fořtová Andrea8,Růžek Daniel8,Nasrallah Gheyath9,Abu-Raddadi Laith9,Al-Sadeq Duaa9,Abdallah Marah9,Lilleri Daniele10,Fornara Chiara10,D'Angelo Piera10,Furione Milena10,Söderlund-Venerm Maria11,Hedman Klaus11,Chochlakis Dimosthenis12,Psaroulaki Anna12,Makridaki Eirini12,Ntoula Artemis12,Rajasekaran John1

Affiliation:

1. Vibrant Sciences LLC

2. Vibrant America LLC

3. Columbia University Medical Center

4. University of Warsaw

5. Medical University of Warsaw

6. University of Texas

7. Royal Liverpool University Hospital

8. Masaryk University

9. Qatar University

10. Fondazione IRCCS Policlinico San Matteo

11. University of Helsinki

12. University of Crete

Abstract

Abstract Tick-borne infections are the most common vector-borne diseases in the USA. Ticks harbor and spread several infections with Lyme disease being the most common tickborne infection in the US and Europe. Lack of awareness about tick populations, specific diagnostic tests, and overlapping symptoms of tick-borne infections can often lead to misdiagnosis affecting treatment and the prevalence data reported especially for non-Lyme tick-borne infections. The diagnostic tests currently available for tick-borne diseases are severely limited in their ability to provide accurate results and cannot detect multiple pathogens in a single run. The multiplex protein microarray developed at Vibrant was designed to detect multiple serological antibodies thereby detecting exposure to multiple pathogens simultaneously. Our microarray in its present form can accommodate 400 antigens and can multiplex across antigen types, whole cell sonicates, recombinant proteins, and peptides. A designed array containing multiple antigens of several microbes including Borrelia burgdorferi, the Lyme disease spirochete, was manufactured and evaluated. The immunoglobulin M (IgM) and G (IgG) responses against several tick-borne microbes and other infectious agents were analyzed for analytical and clinical performance. The microarray improved IgM and IgG sensitivities and specificities of individual microbes when compared with the respective gold standards. The testing was also performed in a single run in comparison to multiple runs needed for comparable testing standards. In summary, our study presents a flexible multiplex microarray platform that can provide quick results with high sensitivity and specificity for evaluating exposure to varied infectious agents especially tick-borne infections.

Publisher

Research Square Platform LLC

Reference61 articles.

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