Hierarchical diversity partitioning of microscopic epibiont community on intertidal molluscan shells and inert surfaces over three geographic regions in Japan

Abstract

Abstract Microscopic epibionts on molluscan shells are a component of the biodiversity of intertidal coastal areas. Because molluscan shells are discrete habitats for the epibiont community, and the molluscan basibionts belong to the local community, epibiont diversity can be evaluated hierarchically by basibiont categories including species. To evaluate the structure of epibiont diversity and effects of taxonomic resolution on the evaluation, epibionts on molluscan shells and inert surfaces were investigated at three geographically distant sites in Japan. In total, 95 species-level taxonomic units of epibionts were obtained from 31 basibiont molluscan species and inert surfaces (plastics and rock chips). The density and the species richness at the site of the lowest latitude were significantly lower than those at the other sites. The epibiont community differred between the three sites, although the major portion of the epibionts were diatoms. Between-site diversity contributed most of the total diversity of the species richness and Simpson diversity in the five levels of the hierarchical partitioning: sample (individual basibiont), basibiont species (molluscan species including rock chips and plastics), surface group (bivalves, chitons + limpets, globose gastropods, and inert surfaces), site, and the total. The taxonomic resolution did not markedly affect the variability of communities between the three sites, although the taxon richness reduced to 51 in the genus-level analysis. The lower taxonomic resolution (genus level) however increased the contribution of the within-sample and decreased the contribution of β diversities at the higher hierarchies, leading to a possible overestimation of biotic homogenization between the communities.