Changes in serum oxylipin profile after one anastomosis gastric bypass

Abstract

Abstract The bioactive oxylipins are important compounds in the chronic inflammation-associated conditions, such as obesity. Treatment of obesity with bariatric surgery improves metabolic state of the patients, thus, the study of oxylipins can provide insight into how the metabolic states of patients changes after the procedure. We determined the free oxylipins profile in serum of 15 patients that underwent one-anastomosis gastric bypass (OAGB) before the surgery, two weeks and 6–9 months after the surgery. For this purpose, a liquid chromatography-tandem mass spectrometry (LC-MS/MS), with solid-phase extraction (SPE) method was validated for the quantification of 27 oxylipins. The analytical method was characterized by good linearity (regression coefficient > 0.99), detection limits from 2 pg to 394 pg. The mean precision was ~ 101%, accuracy was ~ 12% and extraction efficiency for most analytes ranged between 80–120%. In patients serum samples at 6–9 months the oxylipin profiles differed significantly from other time points, mostly with regards to oxylipins associated with endothelial function. Our study demonstrates the value in oxylipin profiling to monitor the metabolic effects of obesity treatment.