Revamping an aquatic irritant to fortune-Utilizing jellyfish for the extraction of hyaluronic acid and its characterization

Abstract

Abstract Hyaluronic acid (HA) is a valuable bioactive polysaccharide that is having numerous application in biomedical and pharmaceutical disciplines. It is normally characterized by the viscous slippery glue which is having a diversified range of relevance. Extraction of hyaluronic acid has been standardized from various sources like pig, rabbit, oxes and human, but these methods have certain limitations like low yield and also require the killing of these animals which is against the experimental ethics. Jellyfish production are now increasing widely and sufficient studies on proper utilization of it is still under progress. This species are known to cause devastation to the fishing vessels, boats and gears. Also the isolation of hyaluronic acid from marine sources are to be exploited further, which is considered to be extensively diverse. Even though numerous methodologies are adopted for its extraction, the impediment lies in the yield and molecular weight of this natural molecule. Hence some minor modification in the existing procedure should be followed. In this present study, isolation and structural characterization was carried out for the HA extracted from jellyfish. Yield obtained is one of the major constrains observed in this study. Further confirmation with FT-IR, Viscosity and Spectrophotometer analysis were also carried out. Protein contamination was not observed as the sample was subjected to SDS PAGE. was done to confirm the presence of any protein contamination. In-vitro moisture absorption studies conducted also indicated a better moisture retention capacity for the extracted compound.