Screening and identification of potato StSPS1, a potential crucial gene regulating seed potato vigor

Abstract

Abstract Backrounds Sucrose phosphate synthase (SPS), as a vital rate-limiting enzyme catalyzing the synthesis of sucrose precursors, plays an indispensable role in the regulation of seed potato vigor. However, the genes encoding SPS in potato have not been fully analyzed, and the main genes affecting seed potato vigor have not been screened and identified. Methods and results In this study, four members of the StSPS family were identified with the potato genome database by homologous gene and conserved domain comparison. Using bioinformatics, the physicochemical properties, subcellular localization, sequence structure, conserved motifs and phylogeny of StSPS were analyzed. The expression levels of StSPS in different potato tissues and from dormancy to sprouting in seed potato were measured using qRT-PCR. The expression data from the potato genome database and previous transcriptome and proteome studies of dormancy to sprouting were compared. Combined with the analysis of SPS enzyme activity in different tuber tissues, the main gene StSPS1 that may regulate seed potato vigor was screened. Moreover, the StSPS1 gene and protein were identified by sequence amplification and prokaryotic expression. Conclusion There are four SPS members in potato, and StSPS1 has been proved to be the main gene encoding sucrose phosphate synthase and regulating the change of seed potato vigor. This study laid a theoretical and experimental foundation for further verifying and clarifying the regulatory function of StSPS1 in the change in seed potato vigor.