Using single cell sequencing to explore the difference in tumor- associated macrophages between left and right colon cancer and evaluate the prognosis of patient

Abstract

This research investigates the role of TAMs in left and right colon cancer progression, utilizing scRNA-seq to identify variances in TAMs and their microenvironment. We gathered macrophages from 12 left and 6 right colon cancer tissue samples, identifying 325 marker genes. Patients were stratified into three subgroups based on the expression of marker gene, which facilitated the examination of tumor microenvironment differences, immune cell infiltration, and immune checkpoint expression differences. WGCNA further identified 27 TAM characteristic genes. Two genes, CXCR4 and RGS2, were selected as prognostic genes through multivariate Cox regression analysis. Using these genes, we have created a TAM-related prognosis evaluation model, which was validated using external datasets. Our findings suggest that TAM-related genes have prognostic significance in colon cancer, offering potential therapeutic targets for left and right colon cancer treatment.