Assessment of Antifungal activity of Probiotics against candidiasis

Abstract

Abstract Aim: To analyse the antifungal effects of probiotics on Candida Albicans Materials and methods: One capsule of commercially available probiotics with 5 billion colony forming units is mixed with 2 ml of sterile water in test tube. Wells are cut in rose Bengal agar, 20µl,50µl,100µl concentration of the solution is placed. Reading was taken after 24hours.Zone of inhibition was noted. Rose Bengal broth, sterilized and 6ml was added in 3 test tubes. Candida suspension was added to all 3-test tube in the range of 5*10 billion CFU/ml.Percentage of dead cells is calculated at wavelength of 600nm at regular time intervals after incubation. Results: In agar well diffusion method, zone of inhibition is 20mm in 25µl, 22mm in 50µl and 25mm for 100µl.Among the concentration 50µl is showed to be effective from MIC activity. Conclusion: Probiotics have good antifungal property against candida albicans. In future this can be also be used in primary and adjuvant therapy in management of oral candidiasis.