lncRNA LINC00652 is a significant prognostic and diagnostic biomarker in the Isfahan Breast cancer patients, correlated to ID1: integrated bioinformatics and experimental approach

Abstract

Abstract Background The primary goal of this study is to compare the levels of expression of ID1, LINC00101, and LINC00652 in Isfahan Breast cancer samples to control samples. Understanding the relationship between variations in RNA expression levels and the clinicopathological statuses of Isfahan patients is another important goal of this research. Method R Studio evaluated 104 breast cancer tissue and 17 control samples in the GSE42568 microarray datasets to identify a relevant dysregulated gene. The limma package was used to conduct differential expression analysis. The DEGs were used to test mRNA-lncRNA interactions using the co-lncRNA online database. The qRT-PCR experiment aimed to determine the degree of expression of ID1, LINC001013, and LINC00652. Result Based on bioinformatics and experimental analyses, ID1 has a significant low-expression in the BC samples. Furthermore, lncRNAs LINC00101 and LINC00652 have significant high expression in the BC samples. ID1 and LINC00652 might be the two potential diagnostic (ROC analysis) and prognostic (Clinicopathological analysis) biomarkers of BC. Discussion According to ROC and clinicopathological analyses, ID1 and LINC00652 could be the two significant prognosis and diagnosis biomarkers in Isfahan Breast cancer patients.