Affiliation:
1. The Herbert Wertheim UF Scripps Institute
2. The Francis Crick Institute
3. MRC Laboratory of Molecular Biology
Abstract
Abstract
With the continuous rise in antibiotic resistance, novel methods that can reveal currently unknown antibiotic resistance mechanisms are essential to prepare and inform health responses. Here we built a library of species representative of the genus Mycobacterium and determined their antibiotic resistance profiles, allowing systematic multispecies comparisons. Analyzing antibiotic resistance in the context of other closely related organisms revealed species with truly exceptional traits, thus providing a solid starting point for the exploration of novel determinants of antibiotic resistance. We illustrate the utility of this genus-level approach to discovery of novel traits by characterizing a previously unrecognized rifamycin-inactivating enzyme that is present in a wide range of bacterial genera.
Publisher
Research Square Platform LLC
Reference61 articles.
1. WHO, W.H.O., Global action plan on antimicrobial resistance. 2015, Geneva, Switzerland: WHO.
2. The antibiotic resistance crisis: part 1: causes and threats;Ventola CL;P T,2015
3. Intrinsic antibiotic resistance: mechanisms, origins, challenges and solutions;Cox G;Int J Med Microbiol,2013
4. Context matters - the complex interplay between resistome genotypes and resistance phenotypes;Dantas G;Curr Opin Microbiol,2012
5. The scourge of antibiotic resistance: the important role of the environment;Finley RL;Clin Infect Dis,2013