Effects of ivermectin on development of Calliphora vicina, Robineau-Desvoidy 1830 (Diptera, Calliphoridae)

Abstract

Abstract Ivermectin is one of the most widely used drugs for parasite control. Previous studies have shown a reduction in the abundance and diversity of "non-target" coprophilous organisms due to the presence of ivermectin (IVM) in bovine faeces (FM). Due to its breadth of behavioural habits, Calliphora vicina is a suitable dipteran species to evaluate the effects of IVM in FM. The aim of this work was to evaluate the effect of five concentrations of IVM in FM (3,000, 300, 100, 30 and 3 ng/g) on the development of C. vicina, having as endpoints: survival, larval, pupal and adult emergence times (% sex) and LC50. Sampling was performed from larval hatching at 60 and 120 min and at 3, 4, 5, 12 hours and every 24 h specimens were weighed until pupae were observed. Data were analysed by ANOVA using a non-parametric Kruskal-Wallis test and as a function of elapsed development time and accumulated degree hours (ADH). Mortality at 3,000 and 300 ng/g was 100% and 97% respectively. There were delays in the development times that were statistically significant in the adult pupation time (p = 0.0216) and in the ADH (p = 0.0431) between the control group (C) and 100 ng/g. The LC50 was determined at 5.6 ng/g. These results demonstrate the lethal and sub-lethal effects of IVM on C. vicina, while highlighting the usefulness of this species as a bio indicator for ecotoxicological studies.