Trichoderma-induced restructuring of the cucumber rhizosphere microbiome and the effect of a synthetic, cross-kingdom microbial community on Fusarium wilt disease and growth in cucumber-Reference-Cited by-同舟云学术

Trichoderma-induced restructuring of the cucumber rhizosphere microbiome and the effect of a synthetic, cross-kingdom microbial community on Fusarium wilt disease and growth in cucumber

Published:2023-10-27 Issue: Volume: Page:
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Author:

Liu Tong¹,Zhang Manman¹,Wang Rui¹,Khan Raja Asad Ali¹,Zhan Xin¹,Ren Sen¹,Jiang Haonan¹,Wu Yinggu¹,Yang Fanxing¹,Yu Xiaoli¹

Affiliation:

1. Hainan University

Abstract

Abstract Trichoderma asperellum FJ035 was introduced into the complex soil microbial community, that included pathogens, present in the soil of a continuous cucumber planting system, to assess the impact of the Trichoderma amendment on the composition of the microbial community and growth and incidence of Fusarium wilt disease caused by Fusarium oxysporum SCCFo1. Results indicated that Trichoderma-induced alterations in the soil microbial community significantly promoted growth and enhanced disease resistance. Additionally, TB11, a cross-kingdom synthetic microbial community consisting of Trichoderma and 30 strains from 11 bacterial genera was constructed. Treatment of SCCFo1-inoculated cucumber plants with TB11 resulted in a 70.0% reduction in Fusarium wilt disease and a 64.59% increase in plant fresh weight compared to control plants. The synthetic community TB11 was then simplified to a TB5 consortium consisting of Trichoderma and 6 strains from 5 bacterial genera. The use of TB5 produced similar benefits in disease control and an even greater growth promotion than was observed withTB11. The bacterial taxa in TB5 directly inhibit the growth of SCCFo1, can solubilize soil nutrients making them more available to cucumber plants and FJ035, and increase the expressionof antioxidant, defense-related enzyme, and growth hormone-related genes in cucumber plants. These findings highlight the potential of utilizing beneficial synthetic microbial assemblies to support sustainable agriculture management systems, and decrease dependence on the use of synthetic chemicals, while boosting crop health and yield.

Publisher

Research Square Platform LLC

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