Cyclin D1·Cdk4 regulates neuronal activity through phosphorylation of GABAA receptors

Author:

Pedraza Neus1,Monserrat Maria Ventura1,Ferrezuelo Francisco1,Torres-Rosell Jordi1,Colomina Neus1,Miguez-Cabello Federico2,Picañol-Párraga Javier2,Soto David2,López-Merino Esperanza3,García-Vilela Celia3,Esteban Jose Antonio3,Egea Joaquim1,GARI ELOI1ORCID

Affiliation:

1. Universitat de Lleida

2. IDIBAPS: Institut d'Investigacions Biomediques August Pi i Sunyer

3. Severo Ochoa Molecular Biology Centre: Universidad Autonoma de Madrid Centro de Biologia Molecular Severo Ochoa

Abstract

Abstract Nuclear Cyclin D1 (Ccnd1) is a main regulator of cell cycle progression and cell proliferation. Interestingly, Ccnd1 moves to the cytoplasm at the onset of differentiation in neuronal precursors. However, the cytoplasmic functions and targets of Ccnd1 in post-mitotic neurons are unknown. Here we identify the α4 subunit of gamma-aminobutyric acid (GABA) type A receptors (GABAARs) as an interactor and target of Ccnd1·Cdk4. Ccnd1 binds to an intracellular loop in α4 and, together with Cdk4, phosphorylates the α4 subunit at threonine 423 and serine 431. These modifications upregulate α4 surface levels and increase the activity of α4-containing GABAARs, measured in whole-cell patch-clamp recordings. In agreement with this role of Ccnd1·Cdk4 in neuronal signaling, inhibition of Cdk4 or expression of the non-phosphorylatable α4 decrease synaptic and extrasynaptic currents in the hippocampus of newborn rats. Moreover, according to α4 functions in synaptic pruning, CCND1 knockout mice display an altered pattern of dendritic spines that is rescued by the phosphomimetic α4. Overall, our findings molecularly link Ccnd1·Cdk4 to GABAARs activity in the central nervous system and highlight a novel role for this G1 cyclin in neuronal signaling.

Publisher

Research Square Platform LLC

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