Identification of key genes and lncRNAs based on M6A methylation regulators and Ferroptosis related genes for the slow flow/no reflow phenomenon

Abstract

Abstract Differentially expressed lncRNAs (DELncs) between treat (STEMI-A and STEMI-B groups) and control group and between STEMI-A (used as control group) and STEMI-B group were obtained by differential analysis. 29 common DELncs were obtained using Venn diagram by intersecting the two DELncs sets. Subsequently, four key lncRNAs based on the treat and control group (ABC-KLncs) with the highest correlation degree (|cor| > 0.8 and p < 0.05) with MMRs and FRGs were obtained, including FAM230B, lnc-EVI2A-3, lnc-PZP-6, and lnc-PLGLB2-5. Through differential analysis and correlation analysis, and 544 DEGs between the treat and control groups with |cor| > 0.3 were identified, and a PPI network was further constructed. MCODE algrithm and Cytohubba algrithm were applied and sifted out four key genes based on the treat and control group (ABC-KGs), consisting of ATP2B3, SUMO2, PPIA, and DNM1. Differential analysis, correlation analysis, PPI network, MCODE algrithm, and Cytohubba algrithm were also performed on the STEMI-A and STEMI-B groups. Moreover, four 4 key lncRNAs based on STEMI-A and STEMI-B groups (AB-KLncs) consisting of LINC00907, lnc-ZNF583-4, RP11-566J3.4, and lnc-USP28-6, and three key genes based the STEMI-A and STEMI-B groups (AB-KGs) consisting of TXN, ASPM, and MYLK were filtered out. In conclusion, this study obtained four ABC-KLncs, four ABC-KGs, four AB-KLncs, and three AB-KGs, these can inform the diagnosis and further study of STEMI as well as the phenomenon of slow flow/no reflow.