Effects of 2.4 GHz radiofrequency electromagnetic field exposure on hippocampal proteins in APP/PS1 mice

Author:

Zhang Shengqingyu1,Wu Xi-Nan2,Li Zhi-qiang2,Mu Yun-Zhen2,Shu Xiao2,Zhou Qiong2,Du Kun-Yu2,Jiang Yu-Rong2,Zhang Yuan3,Wu Hui-Xin2

Affiliation:

1. Kunming Panlong District People's Hospital

2. Kunming Medical University

3. Institute of Biomedical Engineering,Kunming Medical University

Abstract

Abstract

Radiofrequency electromagnetic fields (RF-EMF) can have several biological and genetic effects, particularly in the nervous system. Recent studies have reported that exposure to RF-EMF showed beneficial effects in Alzheimer 's disease (AD). In this study, we determined whether exposure to 2.4 GHz RF-EMF affected protein expression in the hippocampus of APP/PS1 mice. APP/PS1 mice were exposed to 2.4 GHz RF-EMF for 8 hours per day for a total of 90 days. Learning and memory function tests were performed, and protein expression of APP, NR1, NR2A, NR2B, PGC1α, p62 and LC3B in the hippocampus was measured.There was no difference in the learning and memory function between 1-month-old APP/PS1 mice and 1-month-old C57 mice, while 4-month-old APP/PS1 mice showed weaker functions when compared to 1-month-old C57 mice. After 3 months of RF-EMF exposure, the learning and memory function of APP/PS1 and C57 mice improved. The protein expression of APP, NR2A, and NR1 in the hippocampus of 1-month-old APP/PS1 mice increased, while PGC1α expression decreased, and NR2B, p62 and LC3B expression was unaltered. The protein expression of APP, NR2A, NR2B, NR1, PGC1α, and LC3B in the hippocampus of 4-month old APP/PS1 mice increased, while the expression of p62 protein decreased. After 3 months of RF-EMF exposure, the protein expression of NR2A, NR2B, and NR1 protein in the hippocampus of mice decreased, while the expression of PGC1α and p62 increased, and the expression of APP and LC3B was unaltered.

Publisher

Springer Science and Business Media LLC

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